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Specimen Type Blood Container Lavender top tube (EDTA) Alternate Red top tube, plain, no gel Draw Volume 15 mL blood Processed Volume 05 mL serum or EDTA whole blood Collection Routine venipuncture;  Vacutainer Blood Collection S Blood Test Color Page 1 Line 17qq Blood Bottles Geeky Medics Phlebotomy Order Of Draw Explained E Training Vacutainer Blood Collection S And Remended Order Of Draw 6 Mil Clear Poly Tubing What Color For Tsh Blood Test Blood Test Color Page 1 Line 17qq Here is the list of Color codes of the tube, the Additives present in them and their common uses – SNo COLOR CODES ADDITIVE COMMONLY USED FOR 1) Purple / Lavender EDTA (Ethylene diamine tetraacetic acid) CBC, BLOOD TYPING (Rh Factor & ABO Screening), Cross match, Hb, Red cell Indices, ESR by Wintrobe's method etc

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Edta blood bottle colour-Colour Green and purple Blood Culture Fan Aerobic/Anaerobic;Blood Culture Bottle Sets Colour Blue and Purple Blood Culture Aerobic/Anaerobic;

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Blood samples must be taken in the correct order (Order of Draw) Order of Draw Tube Type and Cap Colour Additive Tests Special Instructions 1 Clotting Samples (Blue top) additional Sodium Citrate Clotting studies Tube MUST be filled to the indicated level Call Haematology Ext 451 for bottles 2 Lavender top EDTA FBC 3 Green topEDTA Blood (4 mL) Requires four 4ml EDTA samples To be sent on ice to the laboratory within 15 minutes of collection Overnight fasting necessary Must stop proton pump inhibitors for 2 weeks and H2 antagonists for three days Do not request all gut hormones if only gastrin is necessary Biochemistry Referred Test Barts and the Royal LondonEDTA for crossmatch NAF Oxalate Trace Element EDTA Cap Colour and olume 5ml 3ml 4m l 6ml 2ml 6ml 6ml 25ml 2ml 3m 8ml KFK 469 KFK 333 KFK 304 KFK 3 KFK 501 KFK 321 21G needle KFK 137 23G needle KFK 138 Safety Blood Culture Collection Sets with Blood Culture Bottle Adapter 21G needle KFK 416 23G needle KFK 417 9ml KFK 076

Purple colour blood bottles tubes with EDTA for blood sample drawing $003$007 / Unit Units (Min Order) CN Jiangsu Yuli Medical Instrument Co, Ltd 5 YRS 50 (6) "Friendly service" Contact Supplier,Ltd is located in the industrial concentration district, Shengao Town, Jiangyan District, Taizhou City To its west, it is Jingtai # = Protect specimens from light Electrolytes, Whole Blood None 05hr Pour Plate (submit along with blood culture bottles) Glucose, Whole Blood NaHep 05hr Flow Cytometry Ionized Calcium NaHep 05hr IPLL Lav EDTA 3d Methemoglobin NaHep 05hr CD3/CD4 count;Bottle colour Specific Instructions FBC and film comment EDTA Purple VC or small red if infant Coagulation Screen Sodium citrate blue (VC) or small green coag bottle ifinfant U&Es, creatinine SSTII gold or small orange Li Hep if infant Take by person to lab if high count to avoid cells lysing prior toanalysis Calcium andPhosphate

BD Vacutainer EDTA Blood Collection Tubes From £3240 excl VAT £38 inc VAT View Products See below for stock Becton Dickinson evacuated blood collection tubes are sterile, singleuse and contain tripotassium (K3) or dipotassium (K2) EDTA (Ethylenediamin tetraacetic acid) for whole blood determinations in haematology DescriptionFor guidance on specimen collection or availability of other tests, please bleep the Duty Biochemist on 1417 Biochemistry investigation Sample Container ACE – see angiotensin converting enzyme ACR – see albumin creatinine ratio Adrenocorticotrophic hormone (ACTH) – immediate transport toPaediatric Blood Bottles Lithium Heparin (Green Top/Green Bottles) 13 ml plastic vacutainer with Lithium Heparin preservative and green screwtop lid Unit 1 pack of 10 (average) Qty Porphyrin Kits Kit containing a 24 hour urine collection bottle, faeces pot and EDTA vacutainer all protected from light Patient instructions enclosed in kit

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Do Not use a serum separator tube Special Processing Lab Staff Centrifuge specimen, remove serum/plasma aliquot into aLaboratory Medicine Test Directory If you have any queries regarding the provenance of the displayed reference ranges or the uncertainty of measurement, Laboratory Medicine has data for all quantitative assays which can be provided and discussed by calling the relevant department Uncertainty of measurement is the doubt that exists about thePathology Clinical Services – Tests & Blood Bottles Monovette Colour Description Investigation BIOCHEMISTRY Calcitonin (on ice) Prostate specific antigen (PSA) (or brown bottle) Zinc ( or brown bottle) IMMUNOLOGY Complement white (or brown bottle) (9ml) Amikacin Arbivirus ASO serology (EDTA KBlood ) HAEMATOLOGY Cold agglutains (in

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 Viral blood cultures Lavendertop tube containing 710 mL of blood (EDTA anticoagulant) IV COLLECTION OF ROUTINE BLOOD CULTURES Materials needed BacT Alert Blood Culture Bottles – consists of a FAN (Fastidious Antibiotic Neutralizations) aerobic (green) and a FAN anaerobic bottle (orange)3EDTA (glass) • Spraycoated K 2EDTA (plastic) 8 8 K 2EDTA and K 3EDTA for whole blood hematology determinations K 2EDTA may be used for routine immunohematology testing, and blood donor screening*** Tube inversions ensure mixing of anticoagulant (EDTA) with blood to prevent clotting White • K 2EDTA and gel for plasma separationColour Yellow Blood Culture Paediatric Further information on the specimen containers for specific tests is available in the test library at the base of the Laboratory home page Request Forms

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Whole Blood EDTA Shipping Instructions It is recommended that specimens arrive within 24 hours of draw Draw and package specimen as close to shipping time as possible Necessary Information Date of draw is required Specimen Required Defines the optimal specimen required to perform the test and the preferred volume to complete testingBlood collection tube with color heads 1 PHLEBOTOMY Phlebotomy is the practice of drawing blood from patients and taking the blood specimens to the laboratory to prepare for testing and researches Types of Tubes THE PURPLE ONE (aka "Lavender") These bottles are generally used for haematology tests where whole blood is required for analysis(EU colour code) OR Blood culture Serum / Serum Gel blood1a Citrate blood1b,2 Fluoride / CitrateFluoride blood Heparin / Heparin Gel blood EDTA blood Constriction

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A The differences are the types of closures and the labeling The lavender stopper can either be rubber or a Hemogard ™ closure on a plastic tube Product 3679, a 6 mL plastic tube, and product , a 2 mL plastic tube, both have a distinct pink BD Hemogard ™ closure and product 3685 has a conventional rubber pink stopper The pink stoppered tubes have blood bankNone 24hrs Lactate, Whole Blood NaHep 05hr CD Panel; Blood Bank EDTA Color Pink top Capacity 60 ml Additive K 3 EDTA Common Uses For blood transfusion determinations Special label prompts you to provide required information Additional Information Invert 810x Color Blood Culture Bottles Purple cap & Blue cap Capacity 10 ml blood per bottle recommended

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A Vacutainer blood collection tube is a sterile glass or plastic test tube with a colored rubber stopper creating a vacuum seal inside of the tube, facilitating the drawing of a predetermined volume of liquid Vacutainer tubes may contain additives designed to stabilize and preserve the specimen prior to analytical testing Tubes are available with a safetyengineered stopper, with aThe use of EDTA for measuring cytokines, protein and peptides, and cardiac markers is described, with an outline of the protection of labile molecules provided by this anticoagulant The use of EDTA in proteomics and in general clinical chemistry is also described in comparison with other anticoagulants and with serum samplesTitle Tube guide Author Oxford University Hospitals Created Date 9/6/21 AM

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 Pink Blood Bank (EDTA) Gray (Oxalate/Fluoride) Keeping this in consideration, what do the different colored blood tubes mean?What colour blood bottle is used for Blood film, reticulocytes, Red Cell Folate , HbA1C, monospot for EBV, Parathyroid hormone, FBC, ESR Purple (has EDTA do invert 8X) 2EDTA is a manmade amino acid chelating (binding) Page 8 of 28 agent with an affinity for metals such as lead, mercury, cadmium, and aluminum EDTA's ability to complex is used commercially to either promote or inhibit chemical reactions, depending on application EDTA has been used extensively as a food additive to sequester trace

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STOPPER COLOUR & ORDER OF DRAW TUBE DESCRIPTION COMMON USE SPECIAL INSTRUCTIONS Adult Blood Cultures Blood Culture Aerobic (green) bottle MUST be collected first then Anaerobic (orange) 10 ml blood in each bottle required Paediatric Yellow bottle only 5 ml blood required Mix the blood and media carefully by swirling Use butterfly needleItem VACUETTE® TUBE 4 ml K3E K3EDTA 13x75 lavender capblack ring, nonridged Sample order price inquiry Show details Item VACUETTE® TUBE 6 ml K3E K3EDTA 13x100 pink capblack ring, nonridged Per sales unit 1 Blood Sciences Test Repertoire Table 1 x green if requesting APCR only, otherwise refer to Inherited Thrombophilia screen Part of the thrombophilia screen Can be requested as an individual test if written in the clinical details Discuss with

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Reference Vacutainer 4 Ml Pet Tube For Haematology Tube K2 Edta With Transparent Label And Purple Cap Dd Biolab Laboratory Equipment

 To prevent contamination of the blood samples by the anti –coagulants in the bottles the following order of collection (draw) is advised EDTA Tube – Lavender top 8 – 10 times 7 EDTA Tube – Pink top Blood Samples The colour and size of paediatric tubes vary significantly from standard adult tubes It is therefore ESSENTIALThe tests each bottle is used for are the same the purple one is for cell count, the yellow one is for electrolytes, albumin and LDH, the grey one is for glucose, and blood culture bottles can be used for fluid culturesBlood collection tubes, with EDTA, VACUETTE® EDTA tubes are ideal for the examination of whole blood in haematology and are offered as either K2EDTA or K3EDTA tubes We are currently unable to calculate your contract price for this item List price is being displayed temporarily

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NOTE After the tube has been filled with blood, immediately invert the tube 810 times to mix and ensure adequate anticoagulation of the specimenTo avoid RBC shrinkage due to excess EDTA (with resulting changes in HCT and RBC indices values) and possible dilutional effect, the tubes should be filled with the proper amount of blood for theEDTA BD Vacutainer spraycoated K2EDTA Tubes are used for whole blood hematology determinations and immunohematology testing Closure Reference # Tube Size Draw Volume (mm) (mL) Type Colour Label type 13x75 P 13x75 30 P 13x75 40 P 13x75 40 P 13x100 60 P 16x100 100 STBlood Culture Bottles Blood Culture Ensure aseptic technique Collect Aerobic, then Anaerobic APTT, Coagulation profile, Ddimer, Factor assays, heparin assay, INR, Lupus Blood group EDTA10mL HIV serology Serum SST Blood group Antibody screen EDTA10mL Homocysteine (on ice) EDTA3mL Calcium Serum SST Infectious Disease Serology (IDS) Serum

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Order of draw – in situations of multisampling, it is recommended to arrange the tubes in the following order Tube Volume Determinations Instructions FA Plus () Microbiology To exclude the possibility of septicaemia 1 set of blood culture bottles both aerobic and anaerobic Using a butterfly scalp vein needle only, add 810 mLs blood toColour Cap Ring Tube Contents Tests Special Instructions 1st 3rd 4th 5th 6th 7th 8th 9th Blue KFK 343 35ml EDTA Li Hep Gel IMPORTANT When using a winged Blood Collection Set for these tests, a discard tube (nonadditive KFK 432) Blood Culture bottle adapter 21g (green needle) 24 KFK 416Colour Cat No Tube Type Determinations Blood Cultures Aerobic followed by anaerobic if insufficient blood for both culture bottles, use aerobic bottle only BD BACTEC™ Plus Aerobic medium (, Blue Cap and bottle) BD BACTEC™ Lytic Anaerobic medium (, Purple Cap and bottle)

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It is essential to gently and thoroughly MIX all anticoagulant samples by gently tilting the bottle three or four times Mixing immediately after venepuncture is particularly important for the EDTA (red) bottles When obtaining blood for Coagulation studies or ESR ensure that the bottleAnticoagulant EDTA 04 M at pH about 74 to 80 dissolve 150 g disodium EDTA dihydrate in 700 ml dH2OAdjust pH to 80 adding slowly about 403 ml of 10 M NaOH, fill up to 1SMonovette ® – the revolution in blood collection A blood collection system that combines two blood collection techniques – the aspiration technique and the vacuum technique The SMonovette ® is suitable for all vein conditions and achieves an optimal sample quality, thereby producing the best results The aspiration technique is a gentle technique for routine blood collection

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Blood Tubes G Gold top Gel 35 mL P Purple top EDTA 4 mL Gr Grey top Sodium Fl & EDTA 4 mL B Blue top Trisodium Citrate 27 mL Gn Green top Lithium Heparin 4 mL PK Pink top EDTA 6 mL Tn Tan top nongel 4mL Db Dark Blue Sodium heparin 6 mL Black top Trisodium Citrate 5 mL Contact laboratory Pinktop tube (EDTA) This tube contains EDTA as an anticoagulant These tubes are preferred for blood bank tests NOTE After the tube has been filled with blood, immediately invert the tube 810 times to mix and ensure adequate anticoagulation of the specimenDepartment Tube Type Adults/Paediatrics Tube Colour Chemistry Brown Top (Serum Gel) 49ml Adult Pink Top (EDTA) 34ml Adult Yellow Top (Fluoride) 27ml Adult Yellow Top (Fluoride) 12ml Paediatric Yellow Top (Fluoride) 300µl Neonate Orange Top (Lithium Heparin) 49ml Adult Orange Top (Lithium Heparin) 12ml Paediatric Orange Top (Lithium Heparin) 500µl Neonate

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 Plasma stored from EDTAtreated blood can also be used to measure most proteins, and genetic material can easily be stored from EDTA buffy coats (the interface between the red cells and the plasma after centrifugation, containing white cells and platelets) Note these tubes contain either K2EDTA or K3EDTA2 EDTA Royal Blue Stopper, 6mL Blood Collection Tube Product number 3681 (T1 Metal Free BD Tube EDTA 6mL) for whole blood tests except antimony • Greiner BioOne VACUETTE® TUBE 6 mL NH Trace Elements Sodium Heparin tubes for blood (T819 MetalFree Antimony Only) for antimony blood only

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